Laboratory Test Information Guide
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Committed to Quality

The HRLMP is committed to providing high quality laboratory results for our clients.
As one of the largest integrated laboratory medicine programs in Canada we strive to produce the right results the first time on every sample we receive.
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Test Name:
COVID-19 (Coronavirus disease 2019) PCR
Alternate Name: SARS-CoV-2 virus
Order Entry Module: MIC
LIS Mnemonic: COVID19
Specimen Type: Nasopharyngeal swab (NPS); Endotracheal aspirate (ETA/ETT); sputum (SPU).

If NPS not available, combined throat/nasal swab; deep nasal/mid-nasal turbinate swab (THRNOS)

For the detection of COVID-19 ONLY, order COVID19 (Dovetale: COVID-19 PCR)
As an alternative to a NPS (e.g. patient refusal, frequent repeat testing), a combined oral/deep nasal swab (NASMOU), combined throat/nasal (THRNOS) or deep nasal/mid-nasal turbinate swab (THRNOS) is acceptable.


Collection Information: Samples DO NOT need a Yellow Alert Sticker attached.
Specimens should be collected using McMaster Molecular Media (MMM) and the appropriate swab (with the breakpoint as 80mm): use a flocked nasopharyngeal swab (NPS) swab for NPS specimen, use a flocked nasal-type swab (NS) for other specimen types. The smaller NPS swab can be used to collect to the oral/nasal specimen in younger children. See details in the Memo "Alternative Swabs and Transport Media for COVID-19 testing." Transport immediately at room temperature.
See details on how to collect an oral/deep nasal swab under Educational Resources.

Required Information: A PHOL requisition is NOT required.
Turnaround Time:
Routine:
     within 24 hours


Additional Information: COVID-19 ONLY testing is indicated when a FULL multiplex respiratory panel is not required.
When a FULL respiratory virus panel is required, e.g. high-risk in-patients, pediatric in-patients, order CVIRNPS on NPS and CVIRBAL on BAL specimens. COVID-19 is included in the full respiratory panel and should not be ordered separately.
Cases of COVID-19 will be reported to the Medical Officer of Health.
Positive samples will be tested for Variants of Concern (VOCs) by a laboratory developed PCR assay and selected specimens sent for sequencing, according to algorithms established by Public Health Ontario.

Method of Analysis: Testing for COVID-19 is done by real-time PCR using protocols validated by St. Joseph’s Virology.
Test was Last Updated: 5/17/2021
Testing Laboratory: Virology/Molecular
Testing Site: SJHH
Laboratory Processing Instructions: Samples should be sent to the Virology Laboratory, St Joseph’s Hospital ASAP by routine courier